Determination of Helicobacter pylori resistance to clarithromycin in biopsy samples of gastric mucosa using TaqMan® MGB probes

Objective. To study primary resistance of H. pylori to clarithromycin in residents Gomel region by real-time polymerase chain reaction (PCR RT) using TaqMan® MGB probes. Materials and methods. The included 184 patients diagnosed with gastritis duodenitis, K29, median age 48.5 years (25% 75% were 37 61 old). According the patients’ questionnaires, no clarithromycin-based eradication therapy was administered. determine clarithromycin, a PCR RV method TagMan® probes used. Results. All tested DNA samples positive for Rnase P gene (ICS) considered further analysis (Ct, HEX 20.20-34.14). from cagH FAM 21.26-33.04), indicating infection bacterium, confirmed 152 (82.6%). 23SrRNA (point mutations A2142G A2143G) detected 16 10.5 % Hex 20.24-31.17). control had characteristic curve growth corresponding detection channels; observed negative samples. Conclusion. 10.5%, use triple first-line therapy, including PPIs, amoxicillin as empirical this is consistent Maastricht III-VI recommendations Decree Ministry Health Republic Belarus 01.06.2017 № 54: clinical protocol “Diagnosis treatment digestive diseases.” RT justified prescribe individualized evaluate effectiveness regimens.